Bisulfite-Free Method for Investigating DNA Methylation at Specific Regions
Many commonly used methods to investigate DNA methylation involve bisulfite conversion. However, standard bisulfite-based methods for determining DNA methylation status at specific regions, such as gene promoters, can be time-consuming and expensive when investigating multiple regions and/or high numbers of samples. The OneStep qMethyl system from Zymo Research helps researchers easily asses the DNA methylation status of genes, promoters, or other regions of interest using a bisulfite-free, real-time PCR-based procedure to screen up to 48 clinical or research samples at one time. Integrated DNA Technologies (IDT) recently wrote up a nice article on the OneStep qMethyl system in their DECODED Newsletter. One important application for the OneStep qMethyl system is in the area of biomarker discovery and validation for diseases such as cancer. Did you know that an increase in DNA methylation of promoter regions of key tumor suppressor genes such as RASSF1 and RARß are prevalent in lung, breast, and prostate cancers? Identifying and tracking DNA methylation changes in these promoter regions has shown to be effective for early diagnosis and detection of these cancers. What other ways can you think of to use this bisulfite-free method for DNA methylation analysis in your research?
Image credit: IDT’s DECODED Newsletter, July 2013 issue