Histone ModificationsTools & Technology

Histone Cleavage Can Interfere with the Measurement of Downstream Modifications

Histone modifications are increasingly being used as biomarkers in molecular epidemiology studies. However, a recent report from researchers at Columbia University describes a distinct histone H3 cleavage product, identified by Western blot, in human peripheral blood mononuclear cells (PBMCs), which interferes with the measurement of certain histone modifications (Howe and …