Stem Cell Cultures
Aging, Environment, & DiseaseDNA Methylation and Hydroxymethylation

Differing culture conditions can lead to both reversible and irreversible changes in DNA methylation.

Investigation into the mechanisms of pluripotency in stem cells and their potential applications is ongoing.  It is of utmost importance that these cells be propagated stably, both for the consistency of data within the scientific community and then also for potential clinical applications.  How do culture conditions impact methylation and …

Mouse embryonic stem cells
Developmental Biology & Stem CellsDNA Methylation and Hydroxymethylation

How is DNA Methylation Maintained in ESCs and Differentiated Cells?

DNA methylation has been shown to be very important for development, differentiation, and aging.  But how is DNA methylation maintained?  When and how does de novo DNA methylation occur?  Are there specific DNA methyltransferases (Dnmts) involved in non-symmetric DNA methylation or DNA methylation outside of the CpG context?  There have …