Epigenetic Diagnostics
DNA Methylation and HydroxymethylationTools & Technology

Epigenetic Diagnostics: Locus-specific DNA Methylation Testing Poised for Clinical Impact

Precision medicine holds great potential to make a direct positive impact in the clinic. A growing number of diagnostic panels addressing a list of disease indications are now available for testing. Currently most tests are designed for genetic analysis of sample DNA to help inform treatment decisions. With a few …

ncRNA is Synergistically Regulated by Various Epi-Modifications
Aging, Environment, & DiseaseDNA Methylation and HydroxymethylationHistone ModificationsRegulatory RNATools & Technology

Non-coding RNA Expression is Synergistically modulated by Various Types of Epigenetic Modifications

It is well known that most of the mammalian genome is transcribed, producing large amounts of non-coding RNAs (ncRNAs). The two main types of ncRNA are micro RNAs (miRNAs) and long non-coding RNAs (lncRNAs). MiRNAs are a recently discovered and well-characterized class of ncRNAs that are frequently dysregulated in different …

DNA methylation in the placenta
Developmental Biology & Stem CellsDNA Methylation and HydroxymethylationUncategorized

DNA Methylation in the Placenta: accelerated aging in pregnancy complications

The placenta is a unique organ as it is an extra-embryonic tissue primarily regulated by the fetal genome and shared between mother and fetus. However, it is a transient organ that is only needed throughout pregnancy and gestation and then is discarded after delivery. The essential role of the placenta …

Neuroblastoma cells: nuclei are stained in red, microfilaments are in green and in blue
Aging, Environment, & DiseaseDNA Methylation and HydroxymethylationRegulatory RNA

MicroRNA expression is regulated by DNA methylation: a complex cascade of gene regulation events

MicroRNAs (miRNAs) are non-coding RNAs, roughly 22 nucleotides in size that are central and negative regulators of gene expression. They exert their functions through base-pairing with the 3’UTR of mRNAs   and block expression at the transcriptional and post-transcriptional levels, depending on the perfect or imperfect match in sequences between miRNAs …

DNA repair
Aging, Environment, & DiseaseDNA Methylation and HydroxymethylationHistone Modifications

DNA damage and repair permanently alters chromatin and DNA: Mechanisms of DNA methylation polymorphism

Antonio Pezone, PhD,  Dept Molecular Medicine Antonio Porcellini, MD, PhD, Dept Biology Enrico Vittorio Avvedimento, MD, Dept Molecular Medicine University “Federico II” Naples, Italy DNA methylation is a biological clock that ticks with age, and numerous studies have been able to construct age-prediction models based on methylation status. However in …

Estrogen, Memory, & Aging
Aging, Environment, & DiseaseDNA Methylation and Hydroxymethylation

Estrogen, Memory, & Aging: DNA methylation of the ERα promoter contributes to transcriptional differences in age across the hippocampus

Lara Ianov1,2, Ashok Kumar1, Thomas C. Foster1,2  1Department of Neuroscience, McKnight Brain Institute, 2Genetics and Genomics Program, Genetics Institute, University of Florida, Gainesville, FL 32611 Estradiol (E2) influences a number of processes that are important for maintaining healthy brain function, including memory. The ability of E2 to protect the brain …

5-Formlycytosine (5fC): A new base?
Developmental Biology & Stem CellsDNA Methylation and Hydroxymethylation

5-Formlycytosine (5fC): A new base?

DNA methylation and hydroxymethylation are well-established key players in gene regulation. Although many would agree that 5hmC also plays a critical role, there is still some debate over whether it serves a functional role, or is merely an artifact of DNA de-methylation. Even still, other intermediates of de-methylation are actively …

DNA methylation and hydroxymethylation in the aging brain
Aging, Environment, & DiseaseDNA Methylation and Hydroxymethylation

DNA methylation and hydroxymethylation in the aging brain – Not less, but where

Changes in DNA methylation have long been an area of interest for neurobiology of aging researchers.  With the recent reports of ‘aging’ DNA methylation clocks1,2 and the discovery of roles for methylation3 and hydroxymethylation4 in memory formation5 there is a heightened interest in examining changes in DNA modifications with aging …