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Aging, Environment, & DiseaseDNA Methylation and HydroxymethylationHistone Modifications

NEW LINK FOUND BETWEEN BREAST CANCER AND EPIGENETICS

Breast cancer is one of the most common cancers worldwide in females. Researchers from the University of California, Los Angeles (UCLA) found that PIAS1 is linked to breast tumorigenesis. PIAS1 is a transcriptional repressor that has SUMO (small ubiquitin-like modifier) E3 ligase activity. Recently, it had been explored that PIAS1 …

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bisulfite-conversion
DNA Methylation and HydroxymethylationTools & Technology

Guide to Bisulfite Conversion Kit Selection

Bisulfite conversion remains one of the most powerful and popular methods available to study cytosine methylation in DNA (also known as 5-methylcytosine or 5-mC), and it is widely considered to be the “Gold Standard” for investigating DNA methylation.  To learn more about how the bisulfite conversion process works, check out …

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RNA methylation kit
Regulatory RNATools & Technology

New Kit Available to Study RNA Methylation

While the presence of 5-methylcytosine (5-mC) in RNA has been known for some time, the lack of methods available to study this epigenetic modification has made it challenging to elucidate its function. However, the new EZ RNA Methylation Kit from Zymo Research changes everything.  The EZ RNA Methylation Kit is …

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Bisulfite-Free Methylation Detection
DNA Methylation and HydroxymethylationTools & Technology

Bisulfite-Free Method for Investigating DNA Methylation at Specific Regions

Many commonly used methods to investigate DNA methylation involve bisulfite conversion.  However, standard bisulfite-based methods for determining DNA methylation status at specific regions, such as gene promoters, can be time-consuming and expensive when investigating multiple regions and/or high numbers of samples.  The OneStep qMethyl system from Zymo Research helps researchers …

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DNA Methylation and HydroxymethylationTools & Technology

The Basics of Bisulfite Conversion for DNA Methylation Analysis

Bisulfite conversion is one of the most popular and powerful methods available to study cytosine methylation in DNA (also known as 5-methylcytosine or 5-mC).  The use of sodium bisulfite to convert unmethylated cytosines to uracil was first reported in 1970 by two groups independently, Shapiro et al. at New York …

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DNA Methylation and HydroxymethylationTools & Technology

Everything Starts with Good Primers

As “The Epigenetics Company”, Zymo Research Corporation is dedicated to developing and providing the most comprehensive set of tools for epigenetics research available. With this in mind, we provide on our website the Bisulfite Primer Seeker design program for the quick and easy generation of primers necessary for the PCR …

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Aging, Environment, & DiseaseDevelopmental Biology & Stem CellsDNA Methylation and Hydroxymethylation

Lost in translation? New findings suggest a critical role for cytosine methylation in tRNAs

While cytosine methylation in the DNA context will continue to be an important marker for epigenetic discoveries, current trends in the cytosine methylation game indicate diverse and critical roles of the modification in the RNA context. Much attention has been paid recently to findings in mRNA methylation, suggesting an important …

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Mouse embryonic stem cells
Developmental Biology & Stem CellsDNA Methylation and Hydroxymethylation

How is DNA Methylation Maintained in ESCs and Differentiated Cells?

DNA methylation has been shown to be very important for development, differentiation, and aging.  But how is DNA methylation maintained?  When and how does de novo DNA methylation occur?  Are there specific DNA methyltransferases (Dnmts) involved in non-symmetric DNA methylation or DNA methylation outside of the CpG context?  There have …

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