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Aging, Environment, & DiseaseDNA Methylation and HydroxymethylationTools & Technology

(epi)Genes in Space!

Think back to when you were 17 years old. Most likely, your afternoons did not involve planning experiments that would be conducted aboard the International Space Station (ISS) while you were racing to track practice or chorus rehearsal after school. For Anna-Sophia Boguraev however, this is her reality. In July, …

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DNA Methylation and HydroxymethylationTools & Technology

A Comprehensive Guide to Bisulfite-Converted DNA Amplification and Methylation Sequencing

Over the years, bisulfite conversion has become the most widely used method for DNA methylation analysis. It is the most convenient and effective way to map DNA methylation to individual bases. As the first step in numerous downstream analysis techniques, it is absolutely the bisulfite conversion process is understood and …

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DNA Methylation and HydroxymethylationTools & Technology

The Basics of Bisulfite Conversion for DNA Methylation Analysis

Bisulfite conversion is one of the most popular and powerful methods available to study cytosine methylation in DNA (also known as 5-methylcytosine or 5-mC).  The use of sodium bisulfite to convert unmethylated cytosines to uracil was first reported in 1970 by two groups independently, Shapiro et al. at New York …

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