bisulfite-conversion
DNA Methylation and HydroxymethylationTools & Technology

Guide to Bisulfite Conversion Kit Selection

Bisulfite conversion remains one of the most powerful and popular methods available to study cytosine methylation in DNA (also known as 5-methylcytosine or 5-mC), and it is widely considered to be the “Gold Standard” for investigating DNA methylation.  To learn more about how the bisulfite conversion process works, check out …

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RNA methylation kit
Regulatory RNATools & Technology

New Kit Available to Study RNA Methylation

While the presence of 5-methylcytosine (5-mC) in RNA has been known for some time, the lack of methods available to study this epigenetic modification has made it challenging to elucidate its function. However, the new EZ RNA Methylation Kit from Zymo Research changes everything.  The EZ RNA Methylation Kit is …

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NSun2 and RNA methylation
Regulatory RNATools & Technology

More Methylation in RNA!?! RNA Continues to Surprise…

While DNA methylation is an extensively-studied epigenetic modification, relatively little is known about the methylation of cytosine bases in RNA.  Although the functional consequences of RNA modifications are not clear, RNA molecules are known to be extensively modified.  In fact, there are over 100 reported modifications to RNA, which is …

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dna hydroxymethylation
DNA Methylation and HydroxymethylationTools & Technology

Shining a Light on 5-hmC: Direct Labeling and Optical Detection of DNA Hydroxymethylation

DNA hydroxymethylation is a DNA modification that has been known to exist for decades, but its importance to biology and its role as an epigenetic mark have only been appreciated for the past few years.  The presence of 5-hydroxymethylcytosine (5-hmC) has been observed in the genomes of many different organisms, …

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DNA Methylation and HydroxymethylationTools & Technology

The Basics of Bisulfite Conversion for DNA Methylation Analysis

Bisulfite conversion is one of the most popular and powerful methods available to study cytosine methylation in DNA (also known as 5-methylcytosine or 5-mC).  The use of sodium bisulfite to convert unmethylated cytosines to uracil was first reported in 1970 by two groups independently, Shapiro et al. at New York …

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5-hmC DNA Detection
DNA Methylation and HydroxymethylationTools & Technology

Chop It While It’s Hot! An Enzyme-Free Alternative to Hydroxymethylated DNA Detection

5-hydroxymethylcytosine (5-hmC) has been widely studied in many biological contexts, but the precise mechanistic functions of this epigenetic modification remain largely unknown.  Some evidence suggests that 5-hmC may be an intermediate in the process of DNA demethylation because it can be further oxidized to form 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC), …

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