DNA methylation and hydroxymethylation in the aging brain
Aging, Environment, & DiseaseDNA Methylation and Hydroxymethylation

DNA methylation and hydroxymethylation in the aging brain – Not less, but where

Changes in DNA methylation have long been an area of interest for neurobiology of aging researchers.  With the recent reports of ‘aging’ DNA methylation clocks1,2 and the discovery of roles for methylation3 and hydroxymethylation4 in memory formation5 there is a heightened interest in examining changes in DNA modifications with aging …

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The “neighborhood effect” in chromatin biology: TET proteins-mediated DNA demethylation reconfigures the “histone code” at CpG islands
DNA Methylation and HydroxymethylationHistone Modifications

The “neighborhood effect” in chromatin biology: TET proteins-mediated DNA demethylation reconfigures the “histone code” at CpG islands

A nucleosome is constituted by positively charged histone proteins and negatively charged DNA. Methylation as well as other covalent modifications on DNA and histones ( together also called epigenetic modifications or marks) play important roles in the regulation of chromatin structure and function. TET (Ten-Eleven Translocation) proteins were identified as …

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Imaging technique reveals a unique epigenetic state in mammalian genome
DNA Methylation and HydroxymethylationTools & Technology

Imaging technique reveals a unique epigenetic state in mammalian genome

Epigenetic modifications are chemical processes that affect gene activity without altering the DNA sequencing. The two major DNA epigenetic modifications in the mammalian genome are 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), which are often referred to as the “fifth base” and “sixth base,” respectively. 5mC is generated by DNA methyltransferase (DNMTs) …

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DNA Methylation and Hydroxymethylation

5-methylcytosine and 5-hydroxymethylcytosine Exert Opposite Forces on Base Pairing of DNA Double Helix

     DNA base pairing governs the fundamental function of DNA in life. Importantly, annealing and unwinding of base-paired double helical DNA strands are essential for DNA replication and transcription processes. Moreover, epigenetic DNA base modifications have become recognized to be involved in regulation of DNA at all levels in higher organisms. Our recent research into …

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