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Silencing Diabetes

While Type 1 diabetes (T1D) is an autoimmune disorder characterized by the destruction of pancreatic cells, Type 2 (T2D) is defined as deficient insulin production and impaired insulin action. This is why the two can be distinguished from each other through an antibody screening. The exact cause of β cell …

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Artificial Transcription Factors – A new class of epigenetic drug

Aberrant epigenetic status resulting in dysregulation of gene expression is a hallmark of many cancers and other diseases. In the last decade, countless time and energy has been spent in efforts to develop drugs targeting DNA methylation and histone modifications. To date however, none of these epigenetic drugs are sequence …

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A Comprehensive Guide to Bisulfite-Converted DNA Amplification and Methylation Sequencing

Over the years, bisulfite conversion has become the most widely used method for DNA methylation analysis. It is the most convenient and effective way to map DNA methylation to individual bases. As the first step in numerous downstream analysis techniques, it is absolutely the bisulfite conversion process is understood and …

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An epigenetic platform to study global DNA methylome and transcriptome in parallel from limited tissue such as early embryo

Recent technological advances and the exponential growth of the field of epigenetics, in particular genome-wide studies, are advancing our knowledge and providing more evidence for the interdependence of epigenetic and genetic variations. The importance of genome-wide epigenetic reprogramming in mammalian germ cells and early embryos has shed light on the …

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SNP-SNP here, SNP-SNP there, everywhere a SNP-SNP, old McDonald had a GWAS……

SNP-SNP here, SNP-SNP there, everywhere a SNP-SNP, old McDonald had a GWAS……

Gerhard A. Coetzee, Wange Lu, Peggy Farnham USC Genome-wide association studies (GWAS) have revealed numerous single-nucleotide polymorphisms (SNPs) where allelic frequencies differ between cases and controls of many complex diseases (NHGRI GWA Catalog). In the last decade 6,696 genetic associations of 598 traits have been reported in 16,770 papers (up …

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An incursion into the epigenetic landscape of our ancestors

An incursion into the epigenetic landscape of our ancestors

Studying the evolution of epigenetic regulation in humans has proved to be quite difficult due to the lack of ancient material. Together in collaboration, stem cell biologist, Eran Meshorer and computational biologist, Liran Carmel were able to circumvent this problem by analyzing the genomes of two archaic humans, a Neanderthal …

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Epigenetics, DNA hydroxymethylation, and liver cancer

Somatic Cells Turned Pluripotent by Acid Treatment – Major Breakthrough, Or Too Good To Be True?

The potential use of induced pluripotent stem cells in medicine has the power to completely revolutionize the way physicians treat human diseases.  Imagine the possibility of treating diseased or damaged tissue by taking a patient’s own cells from an unaffected tissue, and turning them into healthy cells to replace the …

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Guide to Bisulfite Conversion Kit Selection

Bisulfite conversion remains one of the most powerful and popular methods available to study cytosine methylation in DNA (also known as 5-methylcytosine or 5-mC), and it is widely considered to be the “Gold Standard” for investigating DNA methylation.  To learn more about how the bisulfite conversion process works, check out …

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