Epigenetic Diagnostics
DNA Methylation and HydroxymethylationTools & Technology

Epigenetic Diagnostics: Locus-specific DNA Methylation Testing Poised for Clinical Impact

Precision medicine holds great potential to make a direct positive impact in the clinic. A growing number of diagnostic panels addressing a list of disease indications are now available for testing. Currently most tests are designed for genetic analysis of sample DNA to help inform treatment decisions. With a few …

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A “Hit and Run” Molecular Model for Lymphoma Development
Aging, Environment, & DiseaseDNA Methylation and Hydroxymethylation

A “Hit and Run” Molecular Model for Lymphoma Development

Diffuse large B-cell lymphoma (DLBCL) represents the most common human lymphoid malignancy, affecting many thousands worldwide each year. Genomic analysis can discriminate lymphomas into two major subtypes with clinical relevance: germinal center B-cells (GCB-like), or activated B-cells (ABC-like).  Current treatment regimens offer only limited overall efficacy as, relative to GCB-like …

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Epigenetic Effects of Folic Acid on Development
Aging, Environment, & DiseaseDNA Methylation and HydroxymethylationImprinting and Inheritance

Maternal Diet Affects DNA Methylation Patterns in Offspring

There is now mounting evidence that diet and other environmental factors can have widespread and long-lasting influences on a developing fetus. Historically, folic acid supplementation is recommended to pregnant mothers as a means to prevent developmental disorders and birth defects. However, there is still no clear understanding how such supplementation …

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Epigenetics of exercise
Article SummariesDNA Methylation and Hydroxymethylation

Exercise: No Pain, No Gain (in DNA Methylation)

Through twin studies and the use of laboratory mice, it is becoming increasingly clear that epigenetic patterns are heavily influenced by the environment. These influences can be either harmful or beneficial to our health. For example, long-term smokers often exhibit characteristic changes in the DNA methylation patterns of key genes …

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DNA Methylation and HydroxymethylationTools & Technology

A State of Dis”array”: Two Recent Studies Highlight Common Problems Associated with Using Illumina’s 450k Array for Epigenetics Research

Epigenetic analysis is one of the hottest areas in all of biological research.  Covalent modifications to DNA, proteins, and RNA, without changing the primary sequence of these molecules, are known to epigenetically regulate numerous cellular processes and contribute to many important human disease phenotypes.  One of the most intensely studied …

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Chromatin StructureHistone ModificationsTools & Technology

You can take a gene out of context, but can you take context out of a gene?

A gene’s precise chromatin context profoundly affects its regulation. For example, genes located near nucleosomes containing acetylated histones are generally actively transcribed. By contrast, genes located near nucleosomes containing histones methylated at specific lysine residues are usually transcriptionally repressed. But is the opposite also true? Do specific gene sequences affect …

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Aging, Environment, & DiseaseChromatin StructureDNA Methylation and HydroxymethylationHistone ModificationsRegulatory RNATools & Technology

Summary of Keystone Symposium: Epigenetic Marks and Cancer Drugs

In order for each of the cells of our bodies to stay healthy, gene expression must be controlled in a tightly regulated fashion. DNA is packaged around histone proteins, and a diverse set of enzymes are responsible for adding or removing specific post-translational modifications, or epigenetic marks, to histone tails. …

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Aging, Environment, & DiseaseDNA Methylation and HydroxymethylationTools & Technology

WHEN IT COMES TO CANCER DRUGS, SOMETIMES LESS IS MORE

The chemotherapeutic drug decitabine (DAC) and its analog azacitidine (AZA) are FDA approved to treat cancers of the blood, such as myelodysplastic syndromes and certain types of leukemias. In high concentrations, DAC and AZA cause acute DNA damage followed by cell death. Currently, both drugs are limited to treatment of …

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