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Epigenetic Diagnostics

Epigenetic Diagnostics: Locus-specific DNA Methylation Testing Poised for Clinical Impact

Precision medicine holds great potential to make a direct positive impact in the clinic. A growing number of diagnostic panels addressing a list of disease indications are now available for testing. Currently most tests are designed for genetic analysis of sample DNA to help inform treatment decisions. With a few …

ncRNA is Synergistically Regulated by Various Epi-Modifications

Non-coding RNA Expression is Synergistically modulated by Various Types of Epigenetic Modifications

It is well known that most of the mammalian genome is transcribed, producing large amounts of non-coding RNAs (ncRNAs). The two main types of ncRNA are micro RNAs (miRNAs) and long non-coding RNAs (lncRNAs). MiRNAs are a recently discovered and well-characterized class of ncRNAs that are frequently dysregulated in different …

DNA methylation in the placenta

DNA Methylation in the Placenta: accelerated aging in pregnancy complications

The placenta is a unique organ as it is an extra-embryonic tissue primarily regulated by the fetal genome and shared between mother and fetus. However, it is a transient organ that is only needed throughout pregnancy and gestation and then is discarded after delivery. The essential role of the placenta …

Neuroblastoma cells: nuclei are stained in red, microfilaments are in green and in blue

MicroRNA expression is regulated by DNA methylation: a complex cascade of gene regulation events

MicroRNAs (miRNAs) are non-coding RNAs, roughly 22 nucleotides in size that are central and negative regulators of gene expression. They exert their functions through base-pairing with the 3’UTR of mRNAs   and block expression at the transcriptional and post-transcriptional levels, depending on the perfect or imperfect match in sequences between miRNAs …

Patient-derived tumor xenograft models (PDXs) for ovarian cancer

A Better Model System for Ovarian Cancer: Patient-derived tumor xenografts (PDXs) are suitable for epigenetic methylome-based cancer research

Ovarian cancer is the most lethal gynecologic malignancy. After epigenomic analysis of patient tumors, aberrant DNA methylation patterns are universally observed in the most abundant histological subtype of ovarian cancer, high-grade serous ovarian cancer (HGSOC). These epigenetic modifications like DNA methylation are known to frequently affect gene regulation involved in …


Hypertriacylglycerolaemia: Genetic and epigenetic factors jointly regulate circulating triacylglycerol levels

Apolipoprotein A5 gene (APOA5) is one of the genes implicated in contributing to a  predisposition for hypertriacylglycerolaemia (HTG),  a complex polygenic pathology that is highly influenced by environment. Hypertriacylglycerolaemia is characterized by having high circulating triacylglycerol levels and is a significant risk factor for cardiovascular disease and atherosclerosis. This genetic …

DNA repair

DNA damage and repair permanently alters chromatin and DNA: Mechanisms of DNA methylation polymorphism

Antonio Pezone, PhD,  Dept Molecular Medicine Antonio Porcellini, MD, PhD, Dept Biology Enrico Vittorio Avvedimento, MD, Dept Molecular Medicine University “Federico II” Naples, Italy DNA methylation is a biological clock that ticks with age, and numerous studies have been able to construct age-prediction models based on methylation status. However in …

Estrogen, Memory, & Aging

Estrogen, Memory, & Aging: DNA methylation of the ERα promoter contributes to transcriptional differences in age across the hippocampus

Lara Ianov1,2, Ashok Kumar1, Thomas C. Foster1,2  1Department of Neuroscience, McKnight Brain Institute, 2Genetics and Genomics Program, Genetics Institute, University of Florida, Gainesville, FL 32611 Estradiol (E2) influences a number of processes that are important for maintaining healthy brain function, including memory. The ability of E2 to protect the brain …